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PCR THERMAL CYCLER

Question asked by vijay1234 in #Coffee Room on Mar 9, 2015
vijay1234
vijay1234 · Mar 9, 2015
Rank E2 - BEGINNER
Project Abstract / Summary : ABSTRACT:
The thermal cycler (also known as a thermo cycler, polymerase chain reaction(PCR) machine or Deoxyribonucleic acid(DNA) amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR).PCR Developed in 1983 by Kary Mullis, PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications. Of all techniques in molecular biology, the PCR, has been the most useful. PCR is a process in which a DNA sequence is artificially amplified by repeated cycles of replication and strand separation, generating thousands to millions of copies of a particular DNA sequence. DNA amplification means making multiple identical copies (replicates) of a DNA sequence. This can be carried out by various different methods, including cell cloning where host cells (manipulated using a vector to contain a DNA insert of interest) are allowed to divide and, as they do so, the insert is replicated also. However, one particular method of DNA amplification has proved very important in recombinant DNA technology and is used in a range of applications in medicine and forensic science. That method is PCR.

Why did you choose to work on this project topic : We are proposing a PCR which is a low-cost, yet accurate thermal cycler, capable of reliably controlling PCR reactions for DNA detection, sequencing, and other applications. We could demonstrate that the described machine is well suited to perform fast reactions with yields comparable to existing macroscopic systems in significantly less time.

Project Category : Electrical / Electronics / Communication
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Institute/College Name: Sanjivani Rular Education Society's College of Engineering ,Kopargaon
City: KOPARGAON
State: Maharastra
Participating Team From: Final Year Posted in: #Coffee Room

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